Ontology highlight
ABSTRACT:
SUBMITTER: Sharma S
PROVIDER: S-EPMC3493422 | biostudies-literature | 2012 Oct
REPOSITORIES: biostudies-literature
Sharma Shweta S Mustafiz Ananda A Singla-Pareek Sneh L SL Shankar Srivastava Prem P Sopory Sudhir Kumar SK
Plant signaling & behavior 20120820 10
As compared with plant system, triose phosphate isomerase (TPI), a crucial enzyme of glycolysis, has been well studied in animals. In order to characterize TPI in plants, a full-length cDNA encoding OscTPI was cloned from rice and expressed in E. coli. The recombinant OscTPI was purified to homogeneity and it showed Km value of 0.1281 ± 0.025 µM, and the Vmax value of 138.7 ± 16 µmol min (-1) mg (-1) which is comparable to the kinetic values studied in other plants. The OscTPI was found to be e ...[more]