Ontology highlight
ABSTRACT:
SUBMITTER: Wei N
PROVIDER: S-EPMC3493533 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Wei Na N Zhang Lei L Huang Huang H Chen Yue Y Zheng Jie J Zhou Xiao X Yi Fan F Du Quan Q Liang Zicai Z
PloS one 20121108 11
It has been noted that target sites located in the coding region or the 3'-untranslated region (3'-UTR) can be silenced to significantly different levels by the same siRNA, but little is known about at what specificity the silencing was achieved. In an exploration of positional effects on siRNA specificity by luciferase reporter system, we surprisingly discovered that siRNA had greatly elevated tolerance towards mismatches in target sites in the 3'-UTR of the mRNA compared with the same target s ...[more]