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Multimodal imaging of human cerebellum - merging X-ray phase microtomography, magnetic resonance microscopy and histology.

ABSTRACT: Imaging modalities including magnetic resonance imaging and X-ray computed tomography are established methods in daily clinical diagnosis of human brain. Clinical equipment does not provide sufficient spatial resolution to obtain morphological information on the cellular level, essential for applying minimally or non-invasive surgical interventions. Therefore, generic data with lateral sub-micrometer resolution have been generated from histological slices post mortem. Sub-cellular spatial resolution, lost in the third dimension as a result of sectioning, is obtained using magnetic resonance microscopy and micro computed tomography. We demonstrate that for human cerebellum grating-based X-ray phase tomography shows complementary contrast to magnetic resonance microscopy and histology. In this study, the contrast-to-noise values of magnetic resonance microscopy and phase tomography were comparable whereas the spatial resolution in phase tomography is an order of magnitude better. The registered data with their complementary information permit the distinct segmentation of tissues within the human cerebellum.

SUBMITTER: Schulz G

PROVIDER: S-EPMC3494013 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Multimodal imaging of human cerebellum - merging X-ray phase microtomography, magnetic resonance microscopy and histology.

Schulz Georg G Waschkies Conny C Pfeiffer Franz F Zanette Irene I Weitkamp Timm T David Christian C Müller Bert B

Scientific reports 20121109

Imaging modalities including magnetic resonance imaging and X-ray computed tomography are established methods in daily clinical diagnosis of human brain. Clinical equipment does not provide sufficient spatial resolution to obtain morphological information on the cellular level, essential for applying minimally or non-invasive surgical interventions. Therefore, generic data with lateral sub-micrometer resolution have been generated from histological slices post mortem. Sub-cellular spatial resolu ...[more]

PMID: 23145319

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Dataset Information

Multimodal imaging of human cerebellum - merging X-ray phase microtomography, magnetic resonance microscopy and histology.

Publications

Multimodal imaging of human cerebellum - merging X-ray phase microtomography, magnetic resonance microscopy and histology.

Similar Datasets

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