Glutathiyl radical as an intermediate in glutathione nitrosation.
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ABSTRACT:
SUBMITTER: Madrasi K
PROVIDER: S-EPMC3494776 | biostudies-literature | 2012 Nov
REPOSITORIES: biostudies-literature
Publications
Glutathiyl radical as an intermediate in glutathione nitrosation.
Madrasi Kumpal K Joshi Mahesh S MS Gadkari Tushar T Kavallieratos Konstantinos K Tsoukias Nikolaos M NM
Free radical biology & medicine 20120822 10
Nitrosation of thiols is thought to be mediated by dinitrogen trioxide (N(2)O(3)) or by nitrogen dioxide radical (()NO(2)). A kinetic study of glutathione (GSH) nitrosation by NO donors in aerated buffered solutions was undertaken. S-nitrosoglutathione (GSNO) formation was assessed spectrophotometrically and by chemiluminescence. The results suggest an increase in the rate of GSNO formation with an increase in GSH with a half-maximum constant EC(50) that depends on NO concentration. Our observed ...[more]
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