Contribution of NS1 effector domain dimerization to influenza A virus replication and virulence.
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ABSTRACT:
SUBMITTER: Ayllon J
PROVIDER: S-EPMC3497675 | biostudies-literature | 2012 Dec
REPOSITORIES: biostudies-literature
Publications
Contribution of NS1 effector domain dimerization to influenza A virus replication and virulence.
Ayllon Juan J Russell Rupert J RJ García-Sastre Adolfo A Hale Benjamin G BG
Journal of virology 20120919 23
Conserved tryptophan-187 facilitates homodimerization of the influenza A virus NS1 protein effector domain. We generated a mutant influenza virus strain expressing NS1-W187R to destabilize this self-interaction. NS1-W187R protein exhibited lower double-stranded RNA (dsRNA)-binding activity, showed a temporal redistribution during infection, and was minimally compromised for interferon antagonism. The mutant virus replicated similarly to the wild type in vitro, but it was slightly attenuated for ...[more]
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