Dataset Information

Extrinsic and intrinsic regulation of DOR/TP53INP2 expression in mice: effects of dietary fat content, tissue type and sex in adipose and muscle tissues.

ABSTRACT:

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Background

DOR/TP53INP2 acts both at the chromosomal level as a nuclear co-factor e.g. for the thyroid hormone receptor and at the extrachromosomal level as an organizing factor of the autophagosome. In a previous study, DOR was shown to be down-regulated in skeletal muscle of obese diabetic Zucker fa/fa rats.

Methods

To identify sites of differential DOR expression in metabolically active tissues, we measured differences in DOR expression in white adipose tissue (WAT), brown adipose tissue (BAT), skeletal muscle (SM) and heart muscle (HM) by qPCR. To assess whether DOR expression is influenced in the short term by nutritional factors, NMRI mice were fed different fat rich diets (fat diet, FD: 18% or high fat diet, HFD: 80% fat) for one week and DOR expression was compared to NMRI mice fed a control diet (normal diet, ND: 3.3% fat). Additionally, DOR expression was measured in young (45?days old) and adult (100?days old) genetically obese (DU6/DU6i) mice and compared to control (DUKs/DUKsi) animals.

Results

ANOVA results demonstrate a significant influence of diet, tissue type and sex on DOR expression in adipose and muscle tissues of FD and HFD mice. In SM, DOR expression was higher in HFD than in FD male mice. In WAT, DOR expression was increased compared to BAT in male FD and HFD mice. In contrast, expression levels in female mice were higher in BAT for both dietary conditions.DOR expression levels in all tissues of 100?days old genetically obese animals were mainly influenced by sex. In HM, DOR expression was higher in male than female animals.

Conclusions

DOR expression varies under the influence of dietary fat content, tissue type and sex. We identified target tissues for further studies to analyze the specific function of DOR in obesity. DOR might be part of a defense mechanism against fat storage in high fat diets or obesity.

SUBMITTER: Fromm-Dornieden C

PROVIDER: S-EPMC3497704 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature

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Publications

Extrinsic and intrinsic regulation of DOR/TP53INP2 expression in mice: effects of dietary fat content, tissue type and sex in adipose and muscle tissues.

Fromm-Dornieden Carolin C Lytovchenko Oleksandr O von der Heyde Silvia S Behnke Nina N Hogl Sebastian S Berghoff Janina J Köpper Frederik F Opitz Lennart L Renne Ulla U Hoeflich Andreas A Beissbarth Tim T Brenig Bertram B Baumgartner Bernhard G BG

Nutrition & metabolism 20120921 1

<h4>Unlabelled</h4><h4>Background</h4>DOR/TP53INP2 acts both at the chromosomal level as a nuclear co-factor e.g. for the thyroid hormone receptor and at the extrachromosomal level as an organizing factor of the autophagosome. In a previous study, DOR was shown to be down-regulated in skeletal muscle of obese diabetic Zucker fa/fa rats.<h4>Methods</h4>To identify sites of differential DOR expression in metabolically active tissues, we measured differences in DOR expression in white adipose tissu ...[more]

PMID: 22995226

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Dataset Information

Extrinsic and intrinsic regulation of DOR/TP53INP2 expression in mice: effects of dietary fat content, tissue type and sex in adipose and muscle tissues.

Unlabelled

Background

Methods

Results

Conclusions

Publications

Extrinsic and intrinsic regulation of DOR/TP53INP2 expression in mice: effects of dietary fat content, tissue type and sex in adipose and muscle tissues.

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