Ontology highlight
ABSTRACT:
SUBMITTER: Daum LT
PROVIDER: S-EPMC3502959 | biostudies-literature | 2012 Dec
REPOSITORIES: biostudies-literature
Daum Luke T LT Rodriguez John D JD Worthy Sue A SA Ismail Nazir A NA Omar Shaheed V SV Dreyer Andries W AW Fourie P Bernard PB Hoosen Anwar A AA Chambers James P JP Fischer Gerald W GW
Journal of clinical microbiology 20120912 12
A novel protocol for full-length Mycobacterium tuberculosis gene analysis of first- and second-line drug resistance was developed using the Ion Torrent Personal Genome Machine (PGM). Five genes-rpoB (rifampin), katG (isoniazid), pncA (pyrazinamide), gyrA (ofloxacin/fluoroquinolone), and rrs (aminoglycosides)-were amplified and sequenced, and results were compared to those obtained by genotypic Hain line probe assay (LPA) and phenotypic Bactec MGIT 960 analysis using 26 geographically diverse Sou ...[more]