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Revisiting global gene expression analysis.

ABSTRACT: Gene expression analysis is a widely used and powerful method for investigating the transcriptional behavior of biological systems, for classifying cell states in disease, and for many other purposes. Recent studies indicate that common assumptions currently embedded in experimental and analytical practices can lead to misinterpretation of global gene expression data. We discuss these assumptions and describe solutions that should minimize erroneous interpretation of gene expression data from multiple analysis platforms.

SUBMITTER: Loven J

PROVIDER: S-EPMC3505597 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature

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Revisiting global gene expression analysis.

Lovén Jakob J Orlando David A DA Sigova Alla A AA Lin Charles Y CY Rahl Peter B PB Burge Christopher B CB Levens David L DL Lee Tong Ihn TI Young Richard A RA

Cell 20121001 3

Gene expression analysis is a widely used and powerful method for investigating the transcriptional behavior of biological systems, for classifying cell states in disease, and for many other purposes. Recent studies indicate that common assumptions currently embedded in experimental and analytical practices can lead to misinterpretation of global gene expression data. We discuss these assumptions and describe solutions that should minimize erroneous interpretation of gene expression data from mu ...[more]

PMID: 23101621

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