Dataset Information

Ultra high-performance liquid chromatography with high-resolution mass spectrometry analysis of African mango (Irvingia gabonensis) seeds, extract, and related dietary supplements.

ABSTRACT: Dietary supplements based on an extract from Irvingia gabonensis (African mango, AM) seeds are one of the popular herbal weight loss dietary supplements in the U.S. market. The extract is believed to be a natural and healthy way to lose weight and improve overall health. However, the chemical composition of AM-based dietary supplements (AMDSs) has never been reported. In this study, the chemical constituents of AM seeds, AM seeds extract (AMSE), and different kinds of commercially available AMDSs have been investigated using an ultra high-performance liquid chromatography with high resolution mass spectrometry method. Ellagic acid, mono-, di-, and tri-O-methyl-ellagic acids, and their glycosides were found as major components in AM seeds. These compounds may be used for quality control of AM extract and related dietary supplements.

SUBMITTER: Sun J

PROVIDER: S-EPMC3506011 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature

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Ultra high-performance liquid chromatography with high-resolution mass spectrometry analysis of African mango (Irvingia gabonensis) seeds, extract, and related dietary supplements.

Sun Jianghao J Chen Pei P

Journal of agricultural and food chemistry 20120823 35

Dietary supplements based on an extract from Irvingia gabonensis (African mango, AM) seeds are one of the popular herbal weight loss dietary supplements in the U.S. market. The extract is believed to be a natural and healthy way to lose weight and improve overall health. However, the chemical composition of AM-based dietary supplements (AMDSs) has never been reported. In this study, the chemical constituents of AM seeds, AM seeds extract (AMSE), and different kinds of commercially available AMDS ...[more]

PMID: 22880691

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Project description:The knowledge about the molecular fraction contributing to white wines oxidative stability is still poorly understood. However, the role of S- and N-containing compounds, like glutathione and other peptides, as a source of reductant in many oxidation reactions, and acting against heavy metals toxicity, or lipid and polyphenol oxidation as ROS-scavenger is today very well established. In that respect, the aim of the present study is to introduce an original analytical tool for the direct determination of the available nucleophilic compounds in white wine under acidic pH conditions. One step derivatization of nucleophiles has been realized directly in wines using 4-methyl-1,2-benzoquinone (4MeQ) as an electrophilic probe. Derivatization conditions considering probe concentration, pH, reaction time, MS ionisation conditions and adducts stability, were optimized using model solutions containing standard sulfur and amino compounds (GSH, Cys, HCys and Ser-Aps-Cys-Asp-Ser, Asp-Met, Met and Glu). Ultra-high-performance liquid chromatography coupled to a quadrupole-time of flight mass spectrometer (UHPLC-QqTOF-MS) analysis of up to 92 white wines from different cultivars (Chardonnay, Sauvignon and Semillon) followed by Multivariate analysis (PLS DA) and Wilcoxon test allowed to isolate up to 141 putative wine relevant nucleophiles. Only 20 of these compounds, essentially thiols, were detectable in samples before derivatization, indicating the importance of the quinone trapping on the revelation of wine unknown nucleophiles. Moreover, annotation using online database (Oligonet, Metlin and KEGG) as well as elementary formula determined by isotopic profile, provided evidence of the presence of amino acids (Val, Leu, Ile, Pro, Trp, Cys and Met) and peptides with important antioxidant properties. The complimentary set of MS/MS spectral data greatly accelerated identification of nucleophiles and enabled peptides sequencing. These results show that probing wines with 4-methyl-1,2-benzoquinone enhances thiols ionisation capacity and gives a better screening of specific S- N- containing functional compounds as part of the white wines antioxidant metabolome.

Dataset Information

Ultra high-performance liquid chromatography with high-resolution mass spectrometry analysis of African mango (Irvingia gabonensis) seeds, extract, and related dietary supplements.

Publications

Ultra high-performance liquid chromatography with high-resolution mass spectrometry analysis of African mango (Irvingia gabonensis) seeds, extract, and related dietary supplements.

Similar Datasets

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