Ontology highlight
ABSTRACT:
SUBMITTER: Hanumantha Rao N
PROVIDER: S-EPMC3507537 | biostudies-literature | 2011 Oct
REPOSITORIES: biostudies-literature
Hanumantha Rao N N Baji Babu P P Rajendra L L Sriraman R R Pang Yuk-Ying S YY Schiller John T JT Srinivasan V A VA
Vaccine 20110729 43
The major capsid protein (L1) of human papillomaviruses (HPV) expressed in heterologous systems assembles into virus-like particles (VLPs). We report cloning and expression of codon optimized HPV L1 genes of the two high-risk HPV types 16 and 18 in methylotropic yeast, Pichia pastoris. The VLPs produced in P. pastoris were subjected to three step purification method involving density gradient centrifugations and size exclusion chromatography. The enriched VLPs were characterized using conformati ...[more]