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LincRNA-p21 suppresses target mRNA translation.

ABSTRACT: Mammalian long intergenic noncoding RNAs (lincRNAs) are best known for modulating transcription. Here we report a posttranscriptional function for lincRNA-p21 as a modulator of translation. Association of the RNA-binding protein HuR with lincRNA-p21 favored the recruitment of let-7/Ago2 to lincRNA-p21, leading to lower lincRNA-p21 stability. Under reduced HuR levels, lincRNA-p21 accumulated in human cervical carcinoma HeLa cells, increasing its association with JUNB and CTNNB1 mRNAs and selectively lowering their translation. With elevated HuR, lincRNA-p21 levels declined, which in turn derepressed JunB and ?-catenin translation and increased the levels of these proteins. We propose that HuR controls translation of a subset of target mRNAs by influencing lincRNA-p21 levels. Our findings uncover a role for lincRNA as a posttranscriptional inhibitor of translation.

SUBMITTER: Yoon JH

PROVIDER: S-EPMC3509343 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature

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LincRNA-p21 suppresses target mRNA translation.

Yoon Je-Hyun JH Abdelmohsen Kotb K Srikantan Subramanya S Yang Xiaoling X Martindale Jennifer L JL De Supriyo S Huarte Maite M Zhan Ming M Becker Kevin G KG Gorospe Myriam M

Molecular cell 20120726 4

Mammalian long intergenic noncoding RNAs (lincRNAs) are best known for modulating transcription. Here we report a posttranscriptional function for lincRNA-p21 as a modulator of translation. Association of the RNA-binding protein HuR with lincRNA-p21 favored the recruitment of let-7/Ago2 to lincRNA-p21, leading to lower lincRNA-p21 stability. Under reduced HuR levels, lincRNA-p21 accumulated in human cervical carcinoma HeLa cells, increasing its association with JUNB and CTNNB1 mRNAs and selectiv ...[more]

PMID: 22841487

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