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Large-scale analysis of high-speed atomic force microscopy data sets using adaptive image processing.


ABSTRACT: Modern high-speed atomic force microscopes generate significant quantities of data in a short amount of time. Each image in the sequence has to be processed quickly and accurately in order to obtain a true representation of the sample and its changes over time. This paper presents an automated, adaptive algorithm for the required processing of AFM images. The algorithm adaptively corrects for both common one-dimensional distortions as well as the most common two-dimensional distortions. This method uses an iterative thresholded processing algorithm for rapid and accurate separation of background and surface topography. This separation prevents artificial bias from topographic features and ensures the best possible coherence between the different images in a sequence. This method is equally applicable to all channels of AFM data, and can process images in seconds.

SUBMITTER: Erickson BW 

PROVIDER: S-EPMC3512124 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Large-scale analysis of high-speed atomic force microscopy data sets using adaptive image processing.

Erickson Blake W BW   Coquoz Séverine S   Adams Jonathan D JD   Burns Daniel J DJ   Fantner Georg E GE  

Beilstein journal of nanotechnology 20121113


Modern high-speed atomic force microscopes generate significant quantities of data in a short amount of time. Each image in the sequence has to be processed quickly and accurately in order to obtain a true representation of the sample and its changes over time. This paper presents an automated, adaptive algorithm for the required processing of AFM images. The algorithm adaptively corrects for both common one-dimensional distortions as well as the most common two-dimensional distortions. This met  ...[more]

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