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Crystallization and first data collection of the putative transfer protein TraN from the Gram-positive conjugative plasmid pIP501.

ABSTRACT: Conjugative plasmid transfer is the most important route for the spread of resistance and virulence genes among bacteria. Consequently, bacteria carrying conjugative plasmids are a substantial threat to human health, especially hospitalized patients. Whilst detailed information about the process has been obtained for Gram-negative type-4 secretion systems, little is known about the corresponding mechanisms in Gram-positive (G+) bacteria. The successful purification and crystallization of the putative transfer protein TraN from the G+ conjugative model plasmid pIP501 of Enterococcus faecalis are presented. Native crystals diffracted to 1.8?Å resolution on a synchrotron beamline. The crystals belonged to space group P2(1), with unit-cell parameters a=32.88, b=54.94, c=57.71?Å, ?=91.89° and two molecules per asymmetric unit.

SUBMITTER: Goessweiner-Mohr N

PROVIDER: S-EPMC3515391 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature

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Crystallization and first data collection of the putative transfer protein TraN from the Gram-positive conjugative plasmid pIP501.

Goessweiner-Mohr Nikolaus N Fercher Christian C Abajy Mohammad Yaser MY Grohmann Elisabeth E Keller Walter W

Acta crystallographica. Section F, Structural biology and crystallization communications 20121030 Pt 11

Conjugative plasmid transfer is the most important route for the spread of resistance and virulence genes among bacteria. Consequently, bacteria carrying conjugative plasmids are a substantial threat to human health, especially hospitalized patients. Whilst detailed information about the process has been obtained for Gram-negative type-4 secretion systems, little is known about the corresponding mechanisms in Gram-positive (G+) bacteria. The successful purification and crystallization of the put ...[more]

PMID: 23143259

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Dataset Information

Crystallization and first data collection of the putative transfer protein TraN from the Gram-positive conjugative plasmid pIP501.

Publications

Crystallization and first data collection of the putative transfer protein TraN from the Gram-positive conjugative plasmid pIP501.

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