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Plasmodium berghei ?p52&p36 parasites develop independent of a parasitophorous vacuole membrane in Huh-7 liver cells.


ABSTRACT: The proteins P52 and P36 are expressed in the sporozoite stage of the murine malaria parasite Plasmodium berghei. ?p52&p36 sporozoites lacking expression of both proteins are severely compromised in their capability to develop into liver stage parasites and abort development soon after invasion; presumably due to the absence of a parasitophorous vacuole membrane (PVM). However, a small proportion of P. berghei ?p52&p36 parasites is capable to fully mature in hepatocytes causing breakthrough blood stage infections. We have studied the maturation of replicating ?p52&p36 parasites in cultured Huh-7 hepatocytes. Approximately 50% of ?p52&p36 parasites developed inside the nucleus of the hepatocyte but did not complete maturation and failed to produce merosomes. In contrast cytosolic ?p52&p36 parasites were able to fully mature and produced infectious merozoites. These ?p52&p36 parasites developed into mature schizonts in the absence of an apparent parasitophorous vacuole membrane as shown by immunofluorescence and electron microscopy. Merozoites derived from these maturing ?p52&p36 liver stages were infectious for C57BL/6 mice.

SUBMITTER: Ploemen IH 

PROVIDER: S-EPMC3515443 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Plasmodium berghei Δp52&p36 parasites develop independent of a parasitophorous vacuole membrane in Huh-7 liver cells.

Ploemen Ivo H J IH   Croes Huib J HJ   van Gemert Geert-Jan J GJ   Wijers-Rouw Mietske M   Hermsen Cornelus C CC   Sauerwein Robert W RW  

PloS one 20121205 12


The proteins P52 and P36 are expressed in the sporozoite stage of the murine malaria parasite Plasmodium berghei. Δp52&p36 sporozoites lacking expression of both proteins are severely compromised in their capability to develop into liver stage parasites and abort development soon after invasion; presumably due to the absence of a parasitophorous vacuole membrane (PVM). However, a small proportion of P. berghei Δp52&p36 parasites is capable to fully mature in hepatocytes causing breakthrough bloo  ...[more]

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