Unknown

Dataset Information

0

A histone deacetylase adjusts transcription kinetics at coding sequences during Candida albicans morphogenesis.


ABSTRACT: Despite their classical role as transcriptional repressors, several histone deacetylases, including the baker's yeast Set3/Hos2 complex (Set3C), facilitate gene expression. In the dimorphic human pathogen Candida albicans, the homologue of the Set3C inhibits the yeast-to-filament transition, but the precise molecular details of this function have remained elusive. Here, we use a combination of ChIP-Seq and RNA-Seq to show that the Set3C acts as a transcriptional co-factor of metabolic and morphogenesis-related genes in C. albicans. Binding of the Set3C correlates with gene expression during fungal morphogenesis; yet, surprisingly, deletion of SET3 leaves the steady-state expression level of most genes unchanged, both during exponential yeast-phase growth and during the yeast-filament transition. Fine temporal resolution of transcription in cells undergoing this transition revealed that the Set3C modulates transient expression changes of key morphogenesis-related genes. These include a transcription factor cluster comprising of NRG1, EFG1, BRG1, and TEC1, which form a regulatory circuit controlling hyphal differentiation. Set3C appears to restrict the factors by modulating their transcription kinetics, and the hyperfilamentous phenotype of SET3-deficient cells can be reverted by mutating the circuit factors. These results indicate that the chromatin status at coding regions represents a dynamic platform influencing transcription kinetics. Moreover, we suggest that transcription at the coding sequence can be transiently decoupled from potentially conflicting promoter information in dynamic environments.

SUBMITTER: Hnisz D 

PROVIDER: S-EPMC3516536 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

altmetric image

Publications

A histone deacetylase adjusts transcription kinetics at coding sequences during Candida albicans morphogenesis.

Hnisz Denes D   Bardet Anaïs F AF   Nobile Clarissa J CJ   Petryshyn Andriy A   Glaser Walter W   Schöck Ulrike U   Stark Alexander A   Kuchler Karl K  

PLoS genetics 20121206 12


Despite their classical role as transcriptional repressors, several histone deacetylases, including the baker's yeast Set3/Hos2 complex (Set3C), facilitate gene expression. In the dimorphic human pathogen Candida albicans, the homologue of the Set3C inhibits the yeast-to-filament transition, but the precise molecular details of this function have remained elusive. Here, we use a combination of ChIP-Seq and RNA-Seq to show that the Set3C acts as a transcriptional co-factor of metabolic and morpho  ...[more]

Similar Datasets

2013-01-01 | GSE38427 | GEO
2013-01-01 | GSE38426 | GEO
2013-01-01 | GSE38425 | GEO
2013-01-01 | E-GEOD-38426 | biostudies-arrayexpress
2013-01-01 | E-GEOD-38425 | biostudies-arrayexpress
| PRJNA167837 | ENA
| S-EPMC4111124 | biostudies-literature
| S-EPMC2869326 | biostudies-literature
| S-EPMC4056552 | biostudies-literature
| S-EPMC117938 | biostudies-literature