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Streptomyces antibioticalis, a Novel Species from a Sanitary Landfill Soil.

ABSTRACT: A novel isolate belonging to the genus Streptomyces, strain SL-4(T), was isolated from soil sample collected from a sanitary landfill, New Delhi, India. The taxonomic status of this isolate was studied by polyphasic approach including morphological, physiological and chemo-taxonomic characterization. Spore chains of SL-4(T) were open loops, hooks or extended spirals of wide diameter (retinaculiperti). The cell wall peptidoglycan of the isolate SL-4(T) contained L,L-diaminopimelic acid, suggesting that the strain has a cell wall of chemotype-I. The polar lipid profile of the isolate was of Type II, with phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides. The 16SrRNA gene sequence similarity between SL-4(T) and its phylogenetic relatives Streptomyces atrovirens NRRLB 16357 (T) (DQ026672), S. albogriseolus NRRLB 1305 (T) (AJ494865), S viridodiastaticus NBRC 13106 (T) (AB184317), S. caelestis NRRL 2418 (T) (X80824), S. flavoviridis NBRC 12772 (T) (AB184842), S. pilosus NBRC 12807 (T) (AB184161) and S. longispororuber NBRC 13488 (T) (AB184440) was 99.65, 99.65, 99.64, 99.23, 99.15, 99.14 and 99.13 % respectively. Subsequent DNA-DNA hybridization experiments with the test strain and its clade members showed 55.27, 44.27, 36.86, and 15.65 % relatedness between SL-4(T) and its relatives S. atrovirens, S. albogriseolus, S. viridodiastaticus and S. longispororuber respectively. The genotypic and phenotypic data was analyzed to verify possibility of the isolate SL-4(T) representing novel member of the genus Streptomyces, for which the name S. antibioticalis is being proposed. The type strain is SL-4(T) (=CCM 7434(T)=MTCC 8588(T)).

SUBMITTER: Khanna M

PROVIDER: S-EPMC3516647 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature

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Streptomyces antibioticalis, a Novel Species from a Sanitary Landfill Soil.

Khanna Monisha M Solanki Renu R

Indian journal of microbiology 20120929 4

A novel isolate belonging to the genus Streptomyces, strain SL-4(T), was isolated from soil sample collected from a sanitary landfill, New Delhi, India. The taxonomic status of this isolate was studied by polyphasic approach including morphological, physiological and chemo-taxonomic characterization. Spore chains of SL-4(T) were open loops, hooks or extended spirals of wide diameter (retinaculiperti). The cell wall peptidoglycan of the isolate SL-4(T) contained L,L-diaminopimelic acid, suggestin ...[more]

PMID: 24293718

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Project description:The successful restoration of well-engineered tailings storage facilities is needed to avoid mine tailings problems. This study characterized the bacterial communities from vegetated and non-vegetated soils from a red gypsum landfill resulting from the industrial extraction of titanium. A set of 275 bacteria was isolated from vegetated soil and non-vegetated soil areas and taxonomically characterized using BOX-PCR. The study also evaluated the ability of a subset of 88 isolated bacteria on their ability to produce plant growth promoting (PGP) traits [indoleacetic acid (IAA) production, phosphate solubilization, and siderophore production] and their tolerance to potentially toxic elements (PTEs). Twenty strains were chosen for further analysis to produce inoculum for birch-challenging experiments. Principal component analysis (PCA) showed that the set of pedological parameters (pH, granulometry, carbon, organic matter, and Mg content) alone explained approximately 40% of the differences between the two soils. The highest density of total culturable bacteria was found in the vegetated soil, and it was much higher than that in the non-vegetated soil. The Actinobacteria phyla dominated the culturable soil community (70% in vegetated soil and 95% in non-vegetated soil), while the phyla Firmicutes (including the genus Bacillus) and Bacteroides (including the genera Pedobacter and Olivibacter) were found only in the vegetated soil fraction. Additional genera (Rhizobium, Variovorax, and Ensifer) were found solely in the vegetated soil. The vegetated soil bacteria harbored the most beneficial PGP bacteria with 12% of the isolates showing three or more PGP traits. The strains with higher metal tolerances in our study were Phyllobacterium sp. WR140 (RO1.15), Phyllobacterium sp. WR140 (R01.34), and Streptomyces sp. (R04.15), all isolated from the vegetated soil. Among the isolates tested in challenging experiments, Phyllobacterium (R01.34) and Streptomyces sp. (R05.33) have the greatest potential to act as PGP rhizobacteria and therefore to be used in the biological restoration of tailings dumps.

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Streptomyces antibioticalis, a Novel Species from a Sanitary Landfill Soil.

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Streptomyces antibioticalis, a Novel Species from a Sanitary Landfill Soil.

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