Ontology highlight
ABSTRACT:
SUBMITTER: Daniele MA
PROVIDER: S-EPMC3516911 | biostudies-literature | 2012 Jul
REPOSITORIES: biostudies-literature
Daniele Michael A MA Bandera Yuriy P YP Sharma Deepti D Rungta Parul P Roeder Ryan R Sehorn Michael G MG Foulger Stephen H SH
Small (Weinheim an der Bergstrasse, Germany) 20120424 13
The isolation of a single type of protein from a complex mixture is vital for the characterization of the function, structure, and interactions of the protein of interest and is typically the most laborious aspect of the protein purification process. In this work, a model system is utilized to show the efficacy of synthesizing a "baited" nanoparticle to capture and recycle enzymes (proteins that catalyze chemical reactions) from crude cell lysate. Enzyme trapping and recycling is illustrated wit ...[more]