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Genetically encoded cyclopropene directs rapid, photoclick-chemistry-mediated protein labeling in mammalian cells.

ABSTRACT: We just click: Genetic incorporation of a cyclopropene amino acid CpK (see scheme) site-specifically into proteins in E.?coli and mammalian cells was achieved using an orthogonal aminoacyl-tRNA synthetase/tRNA(CUA) pair (CpKRS/MbtRNA(CUA)). Cyclopropene exhibited fast reaction kinetics in the photoclick reaction and allowed rapid (ca. 2?min) labeling of proteins.

SUBMITTER: Yu Z 

PROVIDER: S-EPMC3517012 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature

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Genetically encoded cyclopropene directs rapid, photoclick-chemistry-mediated protein labeling in mammalian cells.

Yu Zhipeng Z   Pan Yanchao Y   Wang Zhiyong Z   Wang Jiangyun J   Lin Qing Q  

Angewandte Chemie (International ed. in English) 20120920 42

We just click: Genetic incorporation of a cyclopropene amino acid CpK (see scheme) site-specifically into proteins in E. coli and mammalian cells was achieved using an orthogonal aminoacyl-tRNA synthetase/tRNA(CUA) pair (CpKRS/MbtRNA(CUA)). Cyclopropene exhibited fast reaction kinetics in the photoclick reaction and allowed rapid (ca. 2 min) labeling of proteins. ...[more]

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