Ontology highlight
ABSTRACT:
SUBMITTER: Yu Z
PROVIDER: S-EPMC3517012 | biostudies-literature | 2012 Oct
REPOSITORIES: biostudies-literature
Yu Zhipeng Z Pan Yanchao Y Wang Zhiyong Z Wang Jiangyun J Lin Qing Q
Angewandte Chemie (International ed. in English) 20120920 42
We just click: Genetic incorporation of a cyclopropene amino acid CpK (see scheme) site-specifically into proteins in E. coli and mammalian cells was achieved using an orthogonal aminoacyl-tRNA synthetase/tRNA(CUA) pair (CpKRS/MbtRNA(CUA)). Cyclopropene exhibited fast reaction kinetics in the photoclick reaction and allowed rapid (ca. 2 min) labeling of proteins. ...[more]