Influence of immobilized biomolecules on magnetic bead plug formation and retention in capillary electrophoresis.

ABSTRACT: Significant changes in the formation and retention of magnetic bead plugs in a capillary during electrophoresis were studied, and it was demonstrated that these effects were due to the type of biological molecule immobilized on the surface of these beads. Three biological molecules, an antibody, an oligonucleotide, and alkaline phosphatase (AP), were attached to otherwise identical streptavidin-coated magnetic beads through biotin-avidin binding in order to isolate differences in bead immobilization in a magnetic field resulting from the type of biological molecule immobilized on the bead surface. AP was also attached to the magnetic beads using epoxy groups on the bead surfaces (instead of avidin-biotin binding) to study the impact of immobilization chemistry. The formation and retention

SUBMITTER: Henken RL 

PROVIDER: S-EPMC3517076 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature