Ontology highlight
ABSTRACT:
SUBMITTER: Guo Y
PROVIDER: S-EPMC3518033 | biostudies-literature | 2012 Nov
REPOSITORIES: biostudies-literature
Guo Yanyan Y Yuan Hushan H Rice William L WL Kumar Anand T N AT Goergen Craig J CJ Jokivarsi Kimmo K Josephson Lee L
Journal of the American Chemical Society 20121114 47
We provide a new approach for fluorescent probe design termed "PEG-fluorochrome shielding", where PEGylation enhances quantum yields while blocking troublesome interactions between fluorochromes and biomolecules. To demonstrate PEG-fluorochrome shielding, fluorochrome-bearing peptide probes were synthesized, three without PEG and three with a 5 kDa PEG functional group. In vitro, PEG blocked the interactions of fluorochrome-labeled peptide probes with each other (absorption spectra, self-quenchi ...[more]