Ontology highlight
ABSTRACT:
SUBMITTER: Mullokandov G
PROVIDER: S-EPMC3518396 | biostudies-literature | 2012 Jul
REPOSITORIES: biostudies-literature
Mullokandov Gavriel G Baccarini Alessia A Ruzo Albert A Jayaprakash Anitha D AD Tung Navpreet N Israelow Benjamin B Evans Matthew J MJ Sachidanandam Ravi R Brown Brian D BD
Nature methods 20120701 8
We introduce two large-scale resources for functional analysis of microRNA (miRNA): a decoy library for inhibiting miRNA function and a sensor library for monitoring microRNA activity. To take advantage of the sensor library, we developed a high-throughput assay called Sensor-seq to simultaneously quantify the activity of hundreds of miRNAs. Using this approach, we show that only the most abundant miRNAs in a cell mediate target suppression. Over 60% of detected miRNAs had no discernible activit ...[more]