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Two-photon optogenetics of dendritic spines and neural circuits.

ABSTRACT: We demonstrate a two-photon optogenetic method that generates action potentials in neurons with single-cell precision, using the red-shifted opsin C1V1(T). We applied the method to optically map synaptic circuits in mouse neocortical brain slices and to activate small dendritic regions and individual spines. Using a spatial light modulator, we split the laser beam onto several neurons and performed simultaneous optogenetic activation of selected neurons in three dimensions.

SUBMITTER: Packer AM

PROVIDER: S-EPMC3518588 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature

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Two-photon optogenetics of dendritic spines and neural circuits.

Packer Adam M AM Peterka Darcy S DS Hirtz Jan J JJ Prakash Rohit R Deisseroth Karl K Yuste Rafael R

Nature methods 20121111 12

We demonstrate a two-photon optogenetic method that generates action potentials in neurons with single-cell precision, using the red-shifted opsin C1V1(T). We applied the method to optically map synaptic circuits in mouse neocortical brain slices and to activate small dendritic regions and individual spines. Using a spatial light modulator, we split the laser beam onto several neurons and performed simultaneous optogenetic activation of selected neurons in three dimensions. ...[more]

PMID: 23142873

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