Ontology highlight
ABSTRACT:
SUBMITTER: Chen MH
PROVIDER: S-EPMC3518856 | biostudies-literature | 2011 Aug
REPOSITORIES: biostudies-literature
Chen Min-Hsuan MH Sandberg Daniel J DJ Babu Kunnel R KR Bubis Jose J Surya Arjun A Ramos Lavoisier S LS Zapata Heidi J HJ Galan Jhenny F JF Sandberg Megan N MN Birge Robert R RR Knox Barry E BE
Biochemistry 20110801 32
The role of the extracellular loop region of a short-wavelength sensitive pigment, Xenopus violet cone opsin, is investigated via computational modeling, mutagenesis, and spectroscopy. The computational models predict a complex H-bonding network that stabilizes and connects the EC2-EC3 loop and the N-terminus. Mutations that are predicted to disrupt the H-bonding network are shown to produce visual pigments that do not stably bind chromophore and exhibit properties of a misfolded protein. The po ...[more]