Ontology highlight
ABSTRACT:
SUBMITTER: Yardimci H
PROVIDER: S-EPMC3521859 | biostudies-literature | 2012 Dec
REPOSITORIES: biostudies-literature
Yardimci Hasan H Wang Xindan X Loveland Anna B AB Tappin Inger I Rudner David Z DZ Hurwitz Jerard J van Oijen Antoine M AM Walter Johannes C JC
Nature 20121128 7428
Replicative DNA helicases generally unwind DNA as a single hexamer that encircles and translocates along one strand of the duplex while excluding the complementary strand (known as steric exclusion). By contrast, large T antigen, the replicative DNA helicase of the simian virus 40 (SV40), is reported to function as a pair of stacked hexamers that pumps double-stranded DNA through its central channel while laterally extruding single-stranded DNA. Here we use single-molecule and ensemble assays to ...[more]