Ontology highlight
ABSTRACT:
SUBMITTER: Ranjitkar P
PROVIDER: S-EPMC3523701 | biostudies-literature | 2012 Nov
REPOSITORIES: biostudies-literature
Ranjitkar Pratistha P Perera B Gayani K BG Swaney Danielle L DL Hari Sanjay B SB Larson Eric T ET Krishnamurty Ratika R Merritt Ethan A EA Villén Judit J Maly Dustin J DJ
Journal of the American Chemical Society 20121106 46
Protein kinases are key components of most mammalian signal transduction networks and are therapeutically relevant drug targets. Efforts to study protein kinase function would benefit from new technologies that are able to profile kinases in complex proteomes. Here, we describe active site-directed probes for profiling kinases in whole cell extracts and live cells. These probes contain general ligands that stabilize a specific inactive conformation of the ATP-binding sites of protein kinases, as ...[more]