Native N-terminus nitrophorin 2 from the kissing bug: similarities to and differences from NP2(D1A).
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ABSTRACT:
SUBMITTER: Berry RE
PROVIDER: S-EPMC3523740 | biostudies-literature | 2012 Sep
REPOSITORIES: biostudies-literature
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Native N-terminus nitrophorin 2 from the kissing bug: similarities to and differences from NP2(D1A).
Berry Robert E RE Muthu Dhanasekaran D Shokhireva Tatiana K TK Garrett Sarah A SA Zhang Hongjun H Walker F Ann FA
Chemistry & biodiversity 20120901 9
The first amino acid of mature native nitrophorin 2 is aspartic acid, and when expressed in E. coli, the wild-type gene of the mature protein retains the methionine-0, which is produced by translation of the start codon. This form of NP2, (M0)NP2, has been found to have different properties from its D1A mutant, for which the Met0 is cleaved by the methionine aminopeptidase of E. coli (R. E. Berry, T. K. Shokhireva, I. Filippov, M. N. Shokhirev, H. Zhang, F. A. Walker, Biochemistry 2007, 46, 6830 ...[more]
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