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Merging of confocal and caging technologies: selective three-color communication with profluorescent reporters.

ABSTRACT: Falling apart, on cue: Signaling pathways often display a profound spatiotemporal component that is best studied using light-activatable reagents. Three separate photolabile moieties that can be distinguished based upon their response to three distinct wavelengths (360, 440, and 560?nm) have been synthesized and evaluated. This tri-color system is also applied to imaging in microwells and HeLa cells (see picture).

SUBMITTER: Priestman MA

PROVIDER: S-EPMC3523745 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature

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Merging of confocal and caging technologies: selective three-color communication with profluorescent reporters.

Priestman Melanie A MA Shell Thomas A TA Sun Liang L Lee Hsien-Ming HM Lawrence David S DS

Angewandte Chemie (International ed. in English) 20120627 31

Falling apart, on cue: Signaling pathways often display a profound spatiotemporal component that is best studied using light-activatable reagents. Three separate photolabile moieties that can be distinguished based upon their response to three distinct wavelengths (360, 440, and 560 nm) have been synthesized and evaluated. This tri-color system is also applied to imaging in microwells and HeLa cells (see picture). ...[more]

PMID: 22740297

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