Dataset Information

Molecular detection of Acinetobacter species in lice and keds of domestic animals in Oromia Regional State, Ethiopia.

ABSTRACT: This study was conducted to determine the presence of Acinetobacter and Rickettsia species DNA in lice and Melophagus ovinus (sheep ked) of animals from Oromia Regional State in Ethiopia. From September through November 2011, a total of 207 cattle, 85 sheep, 47 dogs and 16 cats were examined for ectoparasites. Results of morphological identification revealed several species of ectoparasites: Linognathus vituli (L. vituli), Bovicola bovis (B. bovis) and Solenopotes capillatus (S. capillatus) on cattle; B. ovis and Melophagus ovinus (M. ovinus) on sheep; and Heterodoxus spiniger (H. spiniger) on dogs. There was a significantly (p?0.0001) higher prevalence of L. vituli observed in cattle than both S. capillatus and B. bovis. Molecular identification of lice using an 18S rRNA gene analysis confirms the identified lice species by morphological methods. We detected different Acinetobacter species among lice (11.1%) and keds (86.4%) including A. soli in L. vituli of cattle, A. lowffii in M. ovinus of sheep, A. pittii in H. spiniger of dogs, 1 new Acinetobacter spp. in M. ovinus and 2 new Acinetobacter spp. in H. spiniger of dogs using partial rpoB gene sequence analysis. There was a significantly higher prevalence of Acinetobacter spp. in keds than in lice (p?0.00001). Higher percentage of Acinetobacter spp. DNA was detected in H. spiniger than in both B. ovis and L. vituli (p?0.00001). Carbapenemase resistance encoding genes for blaOXA-23, blaOXA-24, blaOXA-58, blaNDM-1 and blaOXA-51 were not found in any lice and keds. These findings suggest that synanthropic animals and their ectoparasites might increase the risk of human exposure to zoonotic pathogens and could be a source for Acinetobacter spp. infections in humans. However, additional epidemiological data are required to determine whether ectoparasites of animals can act as environmental reservoirs and play a role in spreading these bacteria to both animal and human hosts.

SUBMITTER: Kumsa B

PROVIDER: S-EPMC3524130 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Molecular detection of Acinetobacter species in lice and keds of domestic animals in Oromia Regional State, Ethiopia.

Kumsa Bersissa B Socolovschi Cristina C Parola Philippe P Rolain Jean-Marc JM Raoult Didier D

PloS one 20121217 12

This study was conducted to determine the presence of Acinetobacter and Rickettsia species DNA in lice and Melophagus ovinus (sheep ked) of animals from Oromia Regional State in Ethiopia. From September through November 2011, a total of 207 cattle, 85 sheep, 47 dogs and 16 cats were examined for ectoparasites. Results of morphological identification revealed several species of ectoparasites: Linognathus vituli (L. vituli), Bovicola bovis (B. bovis) and Solenopotes capillatus (S. capillatus) on c ...[more]

PMID: 23285015

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Project description:BackgroundAdherence to preventive measures of Coronavirus disease 2019 (COVID-19) was among the means to tackle the transmission of the virus. However, reluctance to implement the recommended preventive measures has been reported to be a major problem everywhere including Oromia Regional State.PurposeThis research was aimed to assess the level of adherence to COVID-19 preventive measures and associated factors in the study area.Participants and methodsCommunity based cross-sectional study was conducted. Sample of 2751 adults aged ≥ 18 years were used for the quantitative study. Also, 20 FGDs and 30 KIIs were conducted in the qualitative approach. The collected data were entered into Epi info version 7.2.0.1 and analyzed using STATA 15. The qualitative data were entered into NVivo version 12 for its organization. Bivariate and multivariable binary logistic regression analyses were conducted to determine the association between the study variables. Odds Ratio with its 95%CI was calculated and P- Value < 0.05 was used as a cut off points to declare the significance.ResultsThe level of adherence to COVID-19 preventive measure was 8.3. Age [AOR, 4.00; 95% CI: 1.50, 10.45], Illiterate AOR, 0.38; 95% CI: 0.15, 0.93], read and write [AOR, 0.26; 95% CI: 0.10, 0.72], attended primary [AOR, 0.30; 95% CI: 0.13, 0.70], occupation (AOR; 95% CI: 0.29, 0.96] and knowledge [AOR, 0.20; 95% CI: 0.01, 0.11] were factors associated with level of adherence to COVID-19 preventive measures. Political context, unemployment, livelihoods, and social events were mentioned as reasons for the poor adherence to COVID-19 preventive measures.ConclusionsThe overall level of adherence to COVID-19 preventive measures in the study area was low. Age, level of education, occupation, and knowledge were factors associated with level of adherence to COVID-19 preventive measures. Activities to increase the adherence to COVID-19 preventive measures should be implemented by the concerned bodies.

Project description:BackgroundEven though health care seeking interventions potentially reduce child mortality from easily treatable diseases, significant numbers of children die without ever reaching a health facility or due to delays in seeking care in Ethiopia. This study aimed to assess health care seeking behavior for common childhood illnesses and associated factors.MethodsA community-based cross-sectional study was conducted in Jeldu District from January to February 2011. A systematic sampling method was used for sample selection. Data were collected from 422 caregivers with under-five children who experienced diseases within six weeks before the survey. Interviewer administered structured and pre-tested questionnaire which were used to collect data. Data entry and cleaning were carried out using Epi Info version 3.5.1 and analyzed using SPSS version 16. Descriptive analysis was done to determine the magnitude of health care seeking behavior. Multivariate logistic regression analyses were performed to identify associated factors.ResultsA total of 422 caregivers of under-five children were participated in the study giving an overall response rate of 97.5%. Three hundred fifteen (74.6%) children sought care from health facilities for all conditions. However, only 55.4% of them were taken to health facilities as first source treatment during their illness and prompt care was also very low (13.7%). Marital status of the caregivers (AOR = 2.84; 95%CI: 1.62-4.98), number of symptoms experienced by the child (AOR = 2.04; 95%CI: 1.24-3.36) and perceived severity of the illness (AOR = 3.20; 95%CI: 1.96-5.22) were predictors of health care seeking behavior.ConclusionHealth care seeking behavior for childhood illnesses was delayed and decision to seek care from health facilities was influenced by worsening of the illnesses. Thus, community level promotion of prompt health care seeking is essential to enhance the health care seeking behavior for child hood illnesses in the locality.

Project description:A survey was undertaken from December 2017 to April 2018 to assess the biosecurity status of 44 commercial chicken farms established in Bishoftu town, Ethiopia, by interviewing farm owners using a structured questionnaire. The obtained data were summarized using frequency tables and analyzed with Pearson's chi-square test and Fischer's exact value using Stata 14 statistical software. From the assessed chicken farms, 31 (70.45%) were located within 0-50 m from the main road, 39 (88.64%) situated 500 m from the nearest farms, and 27 (61.36%) placed within 0-20 m from the residential areas. Forty-one (93.18%) participants disclosed that their employees did not receive training on biosecurity. From the assessed chicken farms, 30 (68.18%) had fences, 40 (90.91%) had footbaths at their gates, 31 (70.45%) prohibited visitors entrance, and 39 (88.64%) did not exchange equipment with other farms. In addition, 26 (59.09%) farms were easily accessed by wild birds, each of 42 (95.45%) farms purchased day-old chicks and feed, and 40 (90.91%) shared trucks with other farms as well. Among the assessed farms, only 2 (4.55%) had signages to restrict people's access, 9 (20.45%) had isolation rooms for diseased chickens, 14 (31.82%) disposed of dead birds properly, and 10 (22.73%) kept various types of records. Occupation (Fischer's exact value = 8.40; P=0.019), previous training (Fischer's exact value = 4.40; P=0.044), source of the premises (χ 2 = 5.50; P=0.019), and farm capacity (Fischer's exact value = 13.50; P=0.002) were found statistically significantly associated with the farm biosecurity status. The farm biosecurity status was found to be good in farms that were run by civil servants, had trained employees, are owned premises, and were of large and medium scales. In conclusion, the higher poor biosecurity status on chicken farms calls for the implementation of good biosecurity practices in each farm as well as the provision of training to the farm owners and their employees.

Project description:BackgroundUndernutrition is cellular imbalance between supply of nutrients, energy and body's demand to ensure growth, maintenance, and specific function. However, there was no study conducted earlier on this topic in East Borena Zone.ObjectiveTo assess the prevalence of undernutrition and associated factors among pregnant women in East Borena Zone, Liban District.MethodA community-based cross-sectional study was conducted on 420 study participants from November 20 to December 2021. The systematic sampling technique and simple random sampling methods were used to select study participants. Data were double entered into Epi-info software version 7 and SPSS version 21 software for analysis. Descriptive statistics were used to describe the characteristics of study participants. Bivariate and multivariable logistic regressions were carried out to identify the association between independent and dependent variables by measuring the adjusted odds ratio and 95% confidence interval. P-values less than 0.05 were considered statistically significant.ResultsPrevalence of undernutrition among pregnant women was about (44.9%) of family monthly income [AOR = 8.72 (4.80, 15.83)], women's decision-making autonomy [AOR = 0.40 (0.19, 0.82)], skipping meal [AOR = 2.62 (1.41, 4.89)], substance use [AOR = 2.01 (1.07, 3.77)], household food insecurity [AOR = 2.01 (1.06, 3.80)], lack of prenatal dietary advice [AOR = 2.73 (1.53, 4.89)], absence of household latrine [AOR = 9.23 (3.48, 24.46)], not participating health development army's meeting at village level [AOR = 3.01 (1.57, 5.72)] and hand washing habit [AOR = 6.55 (3.02, 14.20)] had shown statistically significant association with undernutrition.ConclusionThe prevalence of undernutrition among pregnant women was high income. Women's decision-making autonomy, skipping meals, substances use, household food insecurity, lack of prenatal dietary advice, poor hand washing habit, lack household of latrine, and not participation in health development army's meeting were found to be predictors of the undernutrition.

Project description:BackgroundAzithromycin is a therapeutically" relevant macrolide antibiotic registered on the Essential Medicines List of the World Health Organization. The fact that medicine is selected as an essential drug doesn't mean that it is of good quality. Hence, a continuous quality evaluation of the drug should be mandated to verify that the right medication is available on the market.ObjectiveTo evaluate the quality of Azithromycin Tablets commonly marketed in Adama, and Modjo town, Oromia Regional State, Ethiopia.MethodsAll six brands were subjected to in-vitro quality control tests, which were carried out according to procedures described in the manufacturer's method, the United States Pharmacopeia, and the WHO inspection tool. All quality control parameters were compared by one-way ANOVA. Statistically, significant difference was considered when P<0.05. The in-vitro dissolution profiles of the brands were also compared statistically using the post-hoc Dunnett test, model-independent and model-dependent approaches.ResultsAll of the evaluated brands agreed with WHO visual inspection criteria. All of the tablets achieved the thickness, and diameter test requirements of the manufacturer's specification (±5%). All brands passed the hardness, friability, weight variation, disintegration, identity, and assay tests as stipulated by USP. The dissolution rate was more than 80% in 30 minutes, which was within the USP specification. The model-independent parameters have confirmed that only two brands (2/6) were deemed better brands for interchangeability. Weibull and Korsemeyer's Peppas model were the best release models.ConclusionAll of the evaluated brands passed the quality specification. The model dependent approaches revealed that drug release data fit well to the Weibull, and Korsemeyer's Peppas release models. However, the model-independent parameters have confirmed that only two brands were deemed better brands (2/6) for interchangeability. Due to the dynamic nature of low-quality medications, the Ethiopian Food, and Drug Authority should keep an eye on marketed products to guarantee their quality, especially for drugs like azithromycin for which non-bioequivalence data from the study has revealed a clinical concern.

Dataset Information

Molecular detection of Acinetobacter species in lice and keds of domestic animals in Oromia Regional State, Ethiopia.

Publications

Molecular detection of Acinetobacter species in lice and keds of domestic animals in Oromia Regional State, Ethiopia.

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