Ontology highlight
ABSTRACT:
SUBMITTER: Zhu J
PROVIDER: S-EPMC3525889 | biostudies-literature | 2013 Jan
REPOSITORIES: biostudies-literature
Zhu Jianjie J Chen Lanxin L Mao Yong Y Zhou Huan H Li Rui R Wang Weipeng W
Genetic testing and molecular biomarkers 20121016 1
Allele-specific amplification on the basis of polymerase chain reaction (PCR) has been widely used for single-nucleotide polymorphism (SNP) genotyping. However, the extraction of PCR-compatible genomic DNA from whole blood is usually required. This process is complicated and tedious, and is prone to cause cross-contamination between samples. To facilitate direct PCR amplification from whole blood without the extraction of genomic DNA, we optimized the pH value of PCR solution and the concentrati ...[more]