Ontology highlight
ABSTRACT:
SUBMITTER: Wada T
PROVIDER: S-EPMC3526265 | biostudies-literature | 2012 Dec
REPOSITORIES: biostudies-literature
Wada Tadashi T Hara Masatoshi M Taneda Takuya T Qingfu Cao C Takata Ryouhei R Moro Kanako K Takeda Kei K Kishimoto Takeo T Handa Hiroshi H
Nucleic acids research 20120816 22
Gene downregulation by antisense morpholino oligonucleotides (MOs) is achieved by either hybridization around the translation initiation codon or by targeting the splice donor site. In the present study, an antisense MO method is introduced that uses a 25-mer MO against a region at least 40-nt upstream from a poly(A) tail junction in the 3'-untranslated region (UTR) of maternal mRNA. The MO removed the poly(A) tail and blocked zebrafish cdk9 (zcdk9) mRNA translation, showing functional mimicry b ...[more]