Unknown

Dataset Information

0

Evaluation of matrix-assisted laser desorption/ionization mass spectrometry for second-generation lignin analysis.


ABSTRACT: Matrix-Assisted Laser Desorption/Ionization time-of-flight (MALDI-TOF) mass spectrometry is evaluated as an elucidation tool for structural features and molecular weights estimation of some extracted herbaceous lignins. Optimization of analysis conditions, using a typical organic matrix, namely ?-cyano-4-hydroxycinnamic acid (CHCA), in combination with ?-cyclodextrin, allows efficient ionization of poorly soluble lignin materials and suppression of matrix-related ions background. Analysis of low-mass fragments ions (m/z 100-600) in the positive ion mode offers a "fingerprint" of starting lignins that could be a fine strategy to qualitatively identify principal inter-unit linkages between phenylpropanoid units. The molecular weights of lignins are estimated using size exclusion chromatography and compared to MALDI-TOF-MS profiles. Miscanthus (Miscanthus x giganteus) and Switchgrass (Panicum Virgatum L.) lignins, recovered after a formic acid/acetic acid/water process or aqueous ammonia soaking, are selected as benchmarks for this study.

SUBMITTER: Richel A 

PROVIDER: S-EPMC3528113 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

altmetric image

Publications

Evaluation of matrix-assisted laser desorption/ionization mass spectrometry for second-generation lignin analysis.

Richel Aurore A   Vanderghem Caroline C   Simon Mathilde M   Wathelet Bernard B   Paquot Michel M  

Analytical chemistry insights 20121213


Matrix-Assisted Laser Desorption/Ionization time-of-flight (MALDI-TOF) mass spectrometry is evaluated as an elucidation tool for structural features and molecular weights estimation of some extracted herbaceous lignins. Optimization of analysis conditions, using a typical organic matrix, namely α-cyano-4-hydroxycinnamic acid (CHCA), in combination with α-cyclodextrin, allows efficient ionization of poorly soluble lignin materials and suppression of matrix-related ions background. Analysis of low  ...[more]

Similar Datasets

| S-EPMC2744082 | biostudies-literature
| S-EPMC8284694 | biostudies-literature
| S-EPMC5007172 | biostudies-other
| S-EPMC5512960 | biostudies-other
| S-EPMC3717335 | biostudies-literature
| S-EPMC8096114 | biostudies-literature
| S-EPMC8355924 | biostudies-literature
| S-EPMC9285620 | biostudies-literature
| S-EPMC6787577 | biostudies-literature
| S-EPMC9541130 | biostudies-literature