Ontology highlight
ABSTRACT:
SUBMITTER: Ding F
PROVIDER: S-EPMC3528176 | biostudies-literature | 2012 Mar
REPOSITORIES: biostudies-literature
Ding Fangyuan F Manosas Maria M Spiering Michelle M MM Benkovic Stephen J SJ Bensimon David D Allemand Jean-François JF Croquette Vincent V
Nature methods 20120311 4
High-throughput, low-cost DNA sequencing has emerged as one of the challenges of the postgenomic era. Here we present the proof of concept for a single-molecule platform that allows DNA identification and sequencing. In contrast to most present methods, our scheme is not based on the detection of the fluorescent nucleotides but on DNA hairpin length. By pulling on magnetic beads tethered by a DNA hairpin to the surface, the molecule can be unzipped. In this open state it can hybridize with compl ...[more]