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Novel antiviral activity of baicalein against dengue virus.


ABSTRACT:

Background

Dengue is a serious arboviral disease currently with no effective antiviral therapy or approved vaccine available. Therefore, finding the effective compound against dengue virus (DENV) replication is very important. Among the natural compounds, bioflavonoids derived mainly from plants are of interest because of their biological and medicinal benefits.

Methods

In the present study, antiviral activity of a bioflavonoid, baicalein, was evaluated against different stages of dengue virus type 2 (DENV-2) replication in Vero cells using focus forming unit reduction assay and quantitative RT-PCR.

Results

Baicalein inhibited DENV-2 replication in Vero cells with IC50= 6.46 ?g/mL and SI= 17.8 when added after adsorption to the cells. The IC50 against DENV-2 was 5.39 ?g/mL and SI= 21.3 when cells were treated 5 hours before virus infection and continuously up to 4 days post infection. Baicalein exhibited direct virucidal effect against DENV-2 with IC 50= 1.55 ?g/mL and showed anti-adsorption effect with IC50 = 7.14 ?g/mL.

Conclusions

Findings presented here suggest that baicalein exerts potent antiviral activity against DENV. Baicalein possesses direct virucidal activity against DENV besides its effects against dengue virus adsorption and intracellular replication of DENV-2. Baicalein, hence, should be considered for in vivo evaluation in the development of an effective antiviral compound against DENV.

SUBMITTER: Zandi K 

PROVIDER: S-EPMC3528482 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature

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Publications

Novel antiviral activity of baicalein against dengue virus.

Zandi Keivan K   Zandi Keivan K   Teoh Boon-Teong BT   Sam Sing-Sin SS   Wong Pooi-Fong PF   Mustafa Mohd Rais MR   Abubakar Sazaly S  

BMC complementary and alternative medicine 20121109


<h4>Background</h4>Dengue is a serious arboviral disease currently with no effective antiviral therapy or approved vaccine available. Therefore, finding the effective compound against dengue virus (DENV) replication is very important. Among the natural compounds, bioflavonoids derived mainly from plants are of interest because of their biological and medicinal benefits.<h4>Methods</h4>In the present study, antiviral activity of a bioflavonoid, baicalein, was evaluated against different stages of  ...[more]

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