Microfluidic chambers for monitoring leukocyte trafficking and humanized nano-proresolving medicines interactions.

ABSTRACT: Leukocyte trafficking plays a critical role in determining the progress and resolution of inflammation. Although significant progress has been made in understanding the role of leukocyte activation in inflammation, dissecting the interactions between different leukocyte subpopulations during trafficking is hampered by the complexity of in vivo conditions and the lack of detail of current in vitro assays. To measure the effects of the interactions between neutrophils and monocytes migrating in response to various chemoattractants, at single-cell resolution, we developed a microfluidic platform that replicates critical features of focal inflammation sites. We integrated an elastase assay into the focal chemotactic chambers (FCCs) of our device that enabled us to distinguish between phlogisti

SUBMITTER: Jones CN 

PROVIDER: S-EPMC3528552 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature