Unknown

Dataset Information

0

HSP70 enhances immunosuppressive function of CD4(+)CD25(+)FoxP3(+) T regulatory cells and cytotoxicity in CD4(+)CD25(-) T cells.


ABSTRACT: Human CD4(+)CD25(+)FoxP3(+) T regulatory cells (Tregs) control effector T cells and play a central role in peripheral tolerance and immune homeostasis. Heat shock protein 70 (HSP70) is a major immunomodulatory molecule, but its effect on the functions of Tregs is not well understood. To investigate target-dependent and -independent Treg functions, we studied cytokine expression, regulation of proliferation and cytotoxicity after exposure of Tregs to HSP70. HSP70-treated Tregs significantly inhibited proliferation of CD4(+)CD25(-) target cells and downregulated the secretion of the proinflammatory cytokines IFN-? and TNF-?. By contrast, HSP70 increased the secretion of Treg suppressor cytokines IL-10 and TGF-?. Treatment with HSP70 enhanced the cytotoxic properties of Tregs only to a minor extent (4-fold), but led to stronger responses in CD4(+)CD25(-) cells (42-fold). HSP70-induced modulation of T-cell responses was further enhanced by combined treatment with HSP70 plus IL-2. Treatment of Tregs with HSP70 led to phosphorylation of PI3K/AKT and the MAPKs JNK and p38, but not that of ERK1/2. Exposure of Tregs to specific inhibitors of PI3K/AKT and the MAPKs JNK and p38 reduced the immunosuppressive function of HSP70-treated Tregs as indicated by the modified secretion of specific target cell (IFN-?, TNF-?) and suppressor cytokines (IL-10, TGF-?). Taken together, the data show that HSP70 enhances the suppressive capacity of Tregs to neutralize target immune cells. Thus HSP70-enhanced suppression of Tregs may prevent exaggerated immune responses and may play a major role in maintaining immune homeostasis.

SUBMITTER: Wachstein J 

PROVIDER: S-EPMC3530531 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

altmetric image

Publications

HSP70 enhances immunosuppressive function of CD4(+)CD25(+)FoxP3(+) T regulatory cells and cytotoxicity in CD4(+)CD25(-) T cells.

Wachstein Julian J   Tischer Sabine S   Figueiredo Constanca C   Limbourg Anne A   Falk Christine C   Immenschuh Stephan S   Blasczyk Rainer R   Eiz-Vesper Britta B  

PloS one 20121226 12


Human CD4(+)CD25(+)FoxP3(+) T regulatory cells (Tregs) control effector T cells and play a central role in peripheral tolerance and immune homeostasis. Heat shock protein 70 (HSP70) is a major immunomodulatory molecule, but its effect on the functions of Tregs is not well understood. To investigate target-dependent and -independent Treg functions, we studied cytokine expression, regulation of proliferation and cytotoxicity after exposure of Tregs to HSP70. HSP70-treated Tregs significantly inhib  ...[more]

Similar Datasets

| S-EPMC6708973 | biostudies-literature
| S-EPMC2845330 | biostudies-literature
| S-EPMC4571343 | biostudies-literature
| S-EPMC1975843 | biostudies-literature
| S-EPMC1978217 | biostudies-literature
| S-EPMC1988926 | biostudies-literature
| S-EPMC2118715 | biostudies-literature
| S-EPMC2148309 | biostudies-literature
| S-EPMC2797933 | biostudies-literature
| S-EPMC4002956 | biostudies-literature