Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing.
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ABSTRACT:
SUBMITTER: Bokulich NA
PROVIDER: S-EPMC3531572 | biostudies-literature | 2013 Jan
REPOSITORIES: biostudies-literature
Publications
Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing.
Bokulich Nicholas A NA Subramanian Sathish S Faith Jeremiah J JJ Gevers Dirk D Gordon Jeffrey I JI Knight Rob R Mills David A DA Caporaso J Gregory JG
Nature methods 20121202 1
High-throughput sequencing has revolutionized microbial ecology, but read quality remains a considerable barrier to accurate taxonomy assignment and α-diversity assessment for microbial communities. We demonstrate that high-quality read length and abundance are the primary factors differentiating correct from erroneous reads produced by Illumina GAIIx, HiSeq and MiSeq instruments. We present guidelines for user-defined quality-filtering strategies, enabling efficient extraction of high-quality d ...[more]
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