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Compromised genomic integrity impedes muscle growth after Atrx inactivation.

ABSTRACT: ATR-X syndrome is a severe intellectual disability disorder caused by mutations in the ATRX gene. Many ancillary clinical features are attributed to CNS deficiencies, yet most patients have muscle hypotonia, delayed ambulation, or kyphosis, pointing to an underlying skeletal muscle defect. Here, we identified a cell-intrinsic requirement for Atrx in postnatal muscle growth and regeneration in mice. Mice with skeletal muscle-specific Atrx conditional knockout (Atrx cKO mice) were viable, but by 3 weeks of age presented hallmarks of underdeveloped musculature, including kyphosis, 20% reduction in body mass, and 34% reduction in muscle fiber caliber. Atrx cKO mice also demonstrated a marked regeneration deficit that was not due to fewer resident satellite cells or their inability to terminally differentiate. However, activation of Atrx-null satellite cells from isolated muscle fibers resulted in a 9-fold reduction in myoblast expansion, caused by delayed progression through mid to late S phase. While in S phase, Atrx colocalized specifically to late-replicating chromatin, and its loss resulted in rampant signs of genomic instability. These observations support a model in which Atrx maintains chromatin integrity during the rapid developmental growth of a tissue.

SUBMITTER: Huh MS

PROVIDER: S-EPMC3533543 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature

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Compromised genomic integrity impedes muscle growth after Atrx inactivation.

Huh Michael S MS Price O'Dea Tina T Ouazia Dahmane D McKay Bruce C BC Parise Gianni G Parks Robin J RJ Rudnicki Michael A MA Picketts David J DJ

The Journal of clinical investigation 20121101 12

ATR-X syndrome is a severe intellectual disability disorder caused by mutations in the ATRX gene. Many ancillary clinical features are attributed to CNS deficiencies, yet most patients have muscle hypotonia, delayed ambulation, or kyphosis, pointing to an underlying skeletal muscle defect. Here, we identified a cell-intrinsic requirement for Atrx in postnatal muscle growth and regeneration in mice. Mice with skeletal muscle-specific Atrx conditional knockout (Atrx cKO mice) were viable, but by 3 ...[more]

PMID: 23114596

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Compromised genomic integrity impedes muscle growth after Atrx inactivation.

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Compromised genomic integrity impedes muscle growth after Atrx inactivation.

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