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Induction, rapid fixation and retention of mutations in vegetatively propagated banana.

ABSTRACT: Mutation discovery technologies have enabled the development of reverse genetics for many plant species and allowed sophisticated evaluation of the consequences of mutagenesis. Such methods are relatively straightforward for seed-propagated plants. To develop a platform suitable for vegetatively propagated species, we treated isolated banana shoot apical meristems with the chemical mutagen ethyl methanesulphonate, recovered plantlets and screened for induced mutations. A high density of GC-AT transition mutations were recovered, similar to that reported in seed-propagated polyploids. Through analysis of the inheritance of mutations, we observed that genotypically heterogeneous stem cells resulting from mutagenic treatment are rapidly sorted to fix a single genotype in the meristem. Further, mutant genotypes are stably inherited in subsequent generations. Evaluation of natural nucleotide variation showed the accumulation of potentially deleterious heterozygous alleles, suggesting that mutation induction may uncover recessive traits. This work therefore provides genotypic insights into the fate of totipotent cells after mutagenesis and suggests rapid approaches for mutation-based functional genomics and improvement of vegetatively propagated crops.

SUBMITTER: Jankowicz-Cieslak J

PROVIDER: S-EPMC3533788 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature

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Induction, rapid fixation and retention of mutations in vegetatively propagated banana.

Jankowicz-Cieslak Joanna J Huynh Owen A OA Brozynska Marta M Nakitandwe Joy J Till Bradley J BJ

Plant biotechnology journal 20120829 9

Mutation discovery technologies have enabled the development of reverse genetics for many plant species and allowed sophisticated evaluation of the consequences of mutagenesis. Such methods are relatively straightforward for seed-propagated plants. To develop a platform suitable for vegetatively propagated species, we treated isolated banana shoot apical meristems with the chemical mutagen ethyl methanesulphonate, recovered plantlets and screened for induced mutations. A high density of GC-AT tr ...[more]

PMID: 22928630

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Project description:Many clonal plants have two reproductive patterns, seed propagation and vegetative propagation. By vegetative propagation, plants reproduce the genetically identical offspring with a low mortality, because resources are supplied from the other individuals through interconnected ramets at vegetative-propagated offspring. However, the ramets transport not only resources but also systemic pathogen. Pathogens evolve to establish and spread widely within the plant population. The superinfection, which is defined as the ability that an established pathogen spreads widely by infecting to already-infected individuals with other strains of a pathogen, is important to the evolution of pathogens. We examine the dynamics of plant reproduction and pathogen propagation considering spatial structure and the effect of superinfection on genetic diversity of pathogen by analysis of several models, 1-strain and multiple-strain models, on two-dimensional square lattice. In the analysis of 1-strain model, we derive equilibrium value by mean-field approximation and pair approximation, and its local stability by Routh-Hurwitz stability criterion. In the multiple-strain models, we analyze the dynamics by numerical simulation of mean-field approximation, pair approximation and Monte Carlo simulation. Through the analyses, we show the effect of parameter values to dynamics of models, such as transition of dominant strain of pathogen, competition between plants and pathogens and density of individuals. As a result, (i) The strain with intermediate cost becomes dominant when both superinfection rate and growth rate are low. (ii) The competition between plants and pathogens occurs in the phase of coexistence of various strains by pair approximation and Monte Carlo simulation. (iii) Too high growth rate leads to the decrease of plant population in all models. (iv) Pathogens are easy to maintain their genetic diversity with low superinfection rate. However, if they do not superinfect, the maintenance becomes difficult. (v) When growth rate of plant is low, individuals are very influenced by distant individuals.

Dataset Information

Induction, rapid fixation and retention of mutations in vegetatively propagated banana.

Publications

Induction, rapid fixation and retention of mutations in vegetatively propagated banana.

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