Size-dependent antimicrobial properties of sugar-encapsulated gold nanoparticles synthesized by a green method.
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ABSTRACT:
SUBMITTER: Badwaik VD
PROVIDER: S-EPMC3533927 | biostudies-literature |
REPOSITORIES: biostudies-literature
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Project description:Nowadays, nanoparticles such as gold nanoparticles (Au NPs) with specific biophysical characteristics have attracted remarkable attention as innovative options for the diagnosis and treatment of different diseases. In the present research, Au NPs were green synthesized using the Glaucium flavum leaf extract as an inexpensive and eco-friendly synthesis method. Then, the physicochemical properties were characterized by transmission electron microscopy (TEM), dynamic light scattering method (DLS), scanning electron microscopy (SEM), X-ray diffraction (XRD), Ultraviolet–visible absorption spectroscopy (UV–Vis), Zeta potential, and Fourier transform infrared (FTIR) spectroscopy. Afterwards, the antioxidant capacity was tested and antiviral activity against influenza virus was evaluated by applying TCID50 and PCR assays. The nanoparticles cytotoxicity was tested using the MTT method. The shape and size of Au nanoparticles were modulated by varying leaf concentrations with face-centered cubic (FCC) structure. At higher concentrations, long-time stable spherical nanoparticles were obtained with a mean particle size of 32 nm and low aggregation degree that could simply combine with various bioactive compounds. The outcomes exhibited effective antiviral and antioxidant activities with low cytotoxicity and acceptable biocompatibility of green synthesized Au NPs. The aim of the present study was to develop a potentially environmentally friendly nanoplatform with excellent antiviral and antioxidant functions and acceptable biocompatibility for promising biomedical applications in the future. Supplementary Information The online version contains supplementary material available at 10.1007/s13204-022-02705-1.
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Project description:The pursuit for bioremediation has led to alternative route for the synthesis of nanoparticles and their hybrids. Data in this article display optical properties and progress in the formation of silver and silver/nickel bimetallic nanoparticles using eco-friendly reducing agent (Akinsiku et al. 2018). The as-prepared nanoparticles portrayed nanocrystalline nature as revealed in the x-ray powder diffraction (XRPD) data. Data also exposed antimicrobial activity of the synthesized nanoparticles.
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Project description:This report describes strategies to increase the reactive surfaces of integrated gold nanoparticles (AuNPs) by employing two different types of host materials that do not possess strong electrostatic and/or covalent interactive forces. These composite particles are then utilized as highly reactive and recyclable quasi-homogeneous catalysts in a C-C bond forming reaction. The use of mesoporous TiO2 and poly(N-isopropylacrylamide), PNIPAM, particles allows for the formation of relatively small and large guest AuNPs and provides the greatly improved stability of the resulting composite particles. As these AuNPs are physically incorporated into the mesoporous TiO2 (i.e., supported AuNPs) and PNIPAM particles (i.e., encapsulated AuNPs), their surfaces are maximized to serve as highly reactive catalytic sites. Given their increased physicochemical properties (e.g., stability, dispersity, and surface area), these composite particles exhibit notably high catalytic activity, selectivity, and recyclability in the homocoupling of phenylboronic acid in water and EtOH. Although the small supported AuNPs display slightly faster reaction rates than the large encapsulated AuNPs, the apparent activation energies (Ea) of both composite particles are comparable, implying no obvious correlation with the size of guest AuNPs under the reaction conditions. Investigating the overall physical properties of various composite particles and their catalytic functions, including the reactivity, selectivity, and Ea, can lead to the development of highly practical quasi-homogeneous catalysts in green reaction conditions.
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Project description:PurposeThe leaves and flowering stem of Origanum vulgare contain essential oils, flavonoids, phenolic acids and anthocyanins. We propose a new, simple, one-pot, O. vulgare extract (OVE) mediated green synthesis method of biocompatible gold nanoparticles (AuNPs) possessing improved antioxidant, antimicrobial and plasmonic properties.Materials and methodsDifferent concentrations of OVEs were used to reduce gold ions and to synthetize biocompatible spherical AuNPs. Their morphology and physical properties have been investigated by means of transmission electron microscopy, ultraviolet-visible absorption spectroscopy, photon correlation spectroscopy and Fourier transform infrared spectroscopy, whereas their plasmonic properties have been tested using surface-enhanced Raman spectroscopy (SERS). The antioxidant properties of nanoparticles (NPs) have been evaluated by 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, and the antimicrobial tests were performed using the disk diffusion assay. Their cytotoxicity has been assessed by means of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.ResultsThe experimental results confirmed the successful synthesis of biocompatible, spherical, plasmonic NPs having a mean diameter of ~40 nm and an outstanding aqueous stability. This new class of NPs exhibits a very good antioxidant activity and presents interesting inhibitory effects against Staphylococcus aureus and Candida albicans. Due to their plasmonic properties, AuNPs are used as SERS substrates for the detection of a test molecule (methylene blue) up to a concentration of 10-7 M and a pharmaceutical compound (propranolol) in solution. Cytotoxicity assays revealed that AuNPs are better tolerated by normal human dermal fibroblast cells, while the melanoma cancer cells are more sensitive.ConclusionThe biocompatible AuNPs synthetized using OVEs showed significant bactericidal and antimycotic activities, the most sensitive microorganisms being S. aureus and C. albicans, both commonly involved in various dermatological infections. Moreover, the significant antioxidant effect might recommend their use for protective and/or preventive effect in various skin inflammatory conditions, including the reduction in side effects in dermatological infections. Meanwhile, the as-synthesized biocompatible AuNPs can be successfully used as SERS substrates for the detection of pharmaceutical compounds in aqueous solutions.
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