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Peptide-Modulated Activity Enhancement of Acidic Protease Cathepsin E at Neutral pH.

ABSTRACT: Enzymes are regulated by their activation and inhibition. Enzyme activators can often be effective tools for scientific and medical purposes, although they are more difficult to obtain than inhibitors. Here, using the paired peptide method, we report on protease-cathepsin-E-activating peptides that are obtained at neutral pH. These selected peptides also underwent molecular evolution, after which their cathepsin E activation capability improved. Thus, the activators we obtained could enhance cathepsin-E-induced cancer cell apoptosis, which indicated their potential as cancer drug precursors.

SUBMITTER: Komatsu M

PROVIDER: S-EPMC3534310 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Peptide-Modulated Activity Enhancement of Acidic Protease Cathepsin E at Neutral pH.

Komatsu Masayuki M Biyani Madhu M Ghimire Gautam Sunita S Nishigaki Koichi K

International journal of peptides 20121217

Enzymes are regulated by their activation and inhibition. Enzyme activators can often be effective tools for scientific and medical purposes, although they are more difficult to obtain than inhibitors. Here, using the paired peptide method, we report on protease-cathepsin-E-activating peptides that are obtained at neutral pH. These selected peptides also underwent molecular evolution, after which their cathepsin E activation capability improved. Thus, the activators we obtained could enhance cat ...[more]

PMID: 23365585

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