Ontology highlight
ABSTRACT:
SUBMITTER: Shah NH
PROVIDER: S-EPMC3535263 | biostudies-literature | 2012 Jul
REPOSITORIES: biostudies-literature
Shah Neel H NH Dann Geoffrey P GP Vila-Perelló Miquel M Liu Zhihua Z Muir Tom W TW
Journal of the American Chemical Society 20120702 28
We describe the first systematic study of a family of inteins, the split DnaE inteins from cyanobacteria. By measuring in vivo splicing efficiencies and in vitro kinetics, we demonstrate that several inteins can catalyze protein trans-splicing in tens of seconds rather than hours, as is commonly observed for this autoprocessing protein family. Furthermore, we show that when artificially fused, these inteins can be used for rapid generation of protein α-thioesters for expressed protein ligation. ...[more]