Dataset Information

RNA-Seq analysis implicates dysregulation of the immune system in schizophrenia.

ABSTRACT:

Background

While genome-wide association studies identified some promising candidates for schizophrenia, the majority of risk genes remained unknown. We were interested in testing whether integration gene expression and other functional information could facilitate the identification of susceptibility genes and related biological pathways.

Results

We conducted high throughput sequencing analyses to evaluate mRNA expression in blood samples isolated from 3 schizophrenia patients and 3 healthy controls. We also conducted pooled sequencing of 10 schizophrenic patients and matched controls. Differentially expressed genes were identified by t-test. In the individually sequenced dataset, we identified 198 genes differentially expressed between cases and controls, of them 19 had been verified by the pooled sequencing dataset and 21 reached nominal significance in gene-based association analyses of a genome wide association dataset. Pathway analysis of these differentially expressed genes revealed that they were highly enriched in the immune related pathways. Two genes, S100A8 and TYROBP, had consistent changes in expression in both individual and pooled sequencing datasets and were nominally significant in gene-based association analysis.

Conclusions

Integration of gene expression and pathway analyses with genome-wide association may be an efficient approach to identify risk genes for schizophrenia.

SUBMITTER: Xu J

PROVIDER: S-EPMC3535722 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Publications

RNA-Seq analysis implicates dysregulation of the immune system in schizophrenia.

Xu Junzhe J Sun Jingchun J Chen Jingchun J Wang Lily L Li Anna A Helm Matthew M Dubovsky Steven L SL Bacanu Silviu-Alin SA Zhao Zhongming Z Chen Xiangning X

BMC genomics 20121217

<h4>Background</h4>While genome-wide association studies identified some promising candidates for schizophrenia, the majority of risk genes remained unknown. We were interested in testing whether integration gene expression and other functional information could facilitate the identification of susceptibility genes and related biological pathways.<h4>Results</h4>We conducted high throughput sequencing analyses to evaluate mRNA expression in blood samples isolated from 3 schizophrenia patients an ...[more]

PMID: 23282246

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Dataset Information

RNA-Seq analysis implicates dysregulation of the immune system in schizophrenia.

Background

Results

Conclusions

Publications

RNA-Seq analysis implicates dysregulation of the immune system in schizophrenia.

Similar Datasets

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