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Gemi: PCR primers prediction from multiple alignments.

ABSTRACT: Designing primers and probes for polymerase chain reaction (PCR) is a preliminary and critical step that requires the identification of highly conserved regions in a given set of sequences. This task can be challenging if the targeted sequences display a high level of diversity, as frequently encountered in microbiologic studies. We developed Gemi, an automated, fast, and easy-to-use bioinformatics tool with a user-friendly interface to design primers and probes based on multiple aligned sequences. This tool can be used for the purpose of real-time and conventional PCR and can deal efficiently with large sets of sequences of a large size.

SUBMITTER: Sobhy H

PROVIDER: S-EPMC3535827 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Gemi: PCR primers prediction from multiple alignments.

Sobhy Haitham H Colson Philippe P

Comparative and functional genomics 20121219

Designing primers and probes for polymerase chain reaction (PCR) is a preliminary and critical step that requires the identification of highly conserved regions in a given set of sequences. This task can be challenging if the targeted sequences display a high level of diversity, as frequently encountered in microbiologic studies. We developed Gemi, an automated, fast, and easy-to-use bioinformatics tool with a user-friendly interface to design primers and probes based on multiple aligned sequenc ...[more]

PMID: 23316117

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