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Characterization of OXA-204, a carbapenem-hydrolyzing class D ?-lactamase from Klebsiella pneumoniae.

ABSTRACT: A Klebsiella pneumoniae clinical isolate recovered in Tunisia showed resistance to all ?-lactams and decreased susceptibility to carbapenems. K. pneumoniae 204 expressed the carbapenem-hydrolyzing ?-lactamase OXA-204, differing from OXA-48 by two amino acid substitutions (Gln98His and Thr99Arg) (class D ?-lactamase [DBL] numbering). OXA-48 and OXA-204 shared similar resistance profiles, hydrolyzing carbapenems but sparing broad-spectrum cephalosporins. The bla(OXA-204) gene was located on a ca. 150-kb IncA/C-type plasmid, which also carried the bla(CMY-4) gene. The bla(OXA-204) gene was associated with an ISEcp1 element, whereas the bla(OXA-48) genes are usually associated with IS1999.

SUBMITTER: Potron A

PROVIDER: S-EPMC3535949 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature

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Characterization of OXA-204, a carbapenem-hydrolyzing class D β-lactamase from Klebsiella pneumoniae.

Potron Anaïs A Nordmann Patrice P Poirel Laurent L

Antimicrobial agents and chemotherapy 20121031 1

A Klebsiella pneumoniae clinical isolate recovered in Tunisia showed resistance to all β-lactams and decreased susceptibility to carbapenems. K. pneumoniae 204 expressed the carbapenem-hydrolyzing β-lactamase OXA-204, differing from OXA-48 by two amino acid substitutions (Gln98His and Thr99Arg) (class D β-lactamase [DBL] numbering). OXA-48 and OXA-204 shared similar resistance profiles, hydrolyzing carbapenems but sparing broad-spectrum cephalosporins. The bla(OXA-204) gene was located on a ca. ...[more]

PMID: 23114766

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