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Assessment of real-time PCR assay for detection of Rickettsia spp. and Rickettsia rickettsii in banked clinical samples.

ABSTRACT: Two novel real-time PCR assays were developed for the detection of Rickettsia spp. One assay detects all tested Rickettsia spp.; the other is specific for Rickettsia rickettsii. Evaluation using DNA from human blood and tissue samples showed both assays to be more sensitive than nested PCR assays currently in use at the CDC.

SUBMITTER: Kato CY

PROVIDER: S-EPMC3536194 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature

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Assessment of real-time PCR assay for detection of Rickettsia spp. and Rickettsia rickettsii in banked clinical samples.

Kato Cecilia Y CY Chung Ida H IH Robinson Lauren K LK Austin Amy L AL Dasch Gregory A GA Massung Robert F RF

Journal of clinical microbiology 20121107 1

Two novel real-time PCR assays were developed for the detection of Rickettsia spp. One assay detects all tested Rickettsia spp.; the other is specific for Rickettsia rickettsii. Evaluation using DNA from human blood and tissue samples showed both assays to be more sensitive than nested PCR assays currently in use at the CDC. ...[more]

PMID: 23135935

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