A common progenitor for retinal astrocytes and oligodendrocytes.
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ABSTRACT:
SUBMITTER: Rompani SB
PROVIDER: S-EPMC3536471 | biostudies-literature | 2010 Apr
REPOSITORIES: biostudies-literature
Publications
A common progenitor for retinal astrocytes and oligodendrocytes.
Rompani Santiago B SB Cepko Constance L CL
The Journal of neuroscience : the official journal of the Society for Neuroscience 20100401 14
Developing neural tissue undergoes a period of neurogenesis followed by a period of gliogenesis. The lineage relationships among glial cell types have not been defined for most areas of the nervous system. Here we use retroviruses to label clones of glial cells in the chick retina. We found that almost every clone had both astrocytes and oligodendrocytes. In addition, we discovered a novel glial cell type, with features intermediate between those of astrocytes and oligodendrocytes, which we have ...[more]
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The nonadherent cells from the last O4 dish were harvested by centrifugation, and the cells were resuspended in panning buffer containing GalC, MOG, and O1 supernatant and incubated for 15 minutes at room temperature. The cell suspension was washed and then resuspended in panning buffer containing 20 μg donkey anti-mouse APC for 15 minutes. The cells were washed and resuspended in panning buffer containing propidium iodide (PI). EGFP+ astrocytes were then purified by fluorescence activated cell sorting (FACS). Dead cells were gated out using high PI staining and forward light scatter. Astrocytes were identified based on high EGFP fluorescence and negative APC fluorescence from indirect immunostaining for OL markers GalC, MOG, and O1. 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