Dataset Information

A BioBrick compatible strategy for genetic modification of plants.

ABSTRACT:

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Background

Plant biotechnology can be leveraged to produce food, fuel, medicine, and materials. Standardized methods advocated by the synthetic biology community can accelerate the plant design cycle, ultimately making plant engineering more widely accessible to bioengineers who can contribute diverse creative input to the design process.

Results

This paper presents work done largely by undergraduate students participating in the 2010 International Genetically Engineered Machines (iGEM) competition. Described here is a framework for engineering the model plant Arabidopsis thaliana with standardized, BioBrick compatible vectors and parts available through the Registry of Standard Biological Parts (http://www.partsregistry.org). This system was used to engineer a proof-of-concept plant that exogenously expresses the taste-inverting protein miraculin.

Conclusions

Our work is intended to encourage future iGEM teams and other synthetic biologists to use plants as a genetic chassis. Our workflow simplifies the use of standardized parts in plant systems, allowing the construction and expression of heterologous genes in plants within the timeframe allotted for typical iGEM projects.

SUBMITTER: Boyle PM

PROVIDER: S-EPMC3537565 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature

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Publications

A BioBrick compatible strategy for genetic modification of plants.

Boyle Patrick M PM Burrill Devin R DR Inniss Mara C MC Agapakis Christina M CM Deardon Aaron A DeWerd Jonathan G JG Gedeon Michael A MA Quinn Jacqueline Y JY Paull Morgan L ML Raman Anugraha M AM Theilmann Mark R MR Wang Lu L Winn Julia C JC Medvedik Oliver O Schellenberg Kurt K Haynes Karmella A KA Viel Alain A Brenner Tamara J TJ Church George M GM Shah Jagesh V JV Silver Pamela A PA

Journal of biological engineering 20120620 1

<h4>Unlabelled</h4><h4>Background</h4>Plant biotechnology can be leveraged to produce food, fuel, medicine, and materials. Standardized methods advocated by the synthetic biology community can accelerate the plant design cycle, ultimately making plant engineering more widely accessible to bioengineers who can contribute diverse creative input to the design process.<h4>Results</h4>This paper presents work done largely by undergraduate students participating in the 2010 International Genetically E ...[more]

PMID: 22716313

Similar Datasets

Project description:BackgroundBioBrick standard biological parts are designed to make biological systems easier to engineer (e.g. assemble, manipulate, and modify). There are over 5,000 parts available in the Registry of Standard Biological Parts that can be easily assembled into genetic circuits using a standard assembly technique. The standardization of the assembly technique has allowed for wide distribution to a large number of users -- the parts are reusable and interchangeable during the assembly process. The standard assembly process, however, has some limitations. In particular it does not allow for modification of already assembled biological circuits, addition of protein tags to pre-existing BioBrick parts, or addition of non-BioBrick parts to assemblies.ResultsIn this paper we describe a simple technique for rapid generation of synthetic biological circuits using introduction of customized inserts. We demonstrate its use in Escherichia coli (E. coli) to express green fluorescent protein (GFP) at pre-calculated relative levels and to add an N-terminal tag to GFP. The technique uses a new BioBrick part (called a BioScaffold) that can be inserted into cloning vectors and excised from them to leave a gap into which other DNA elements can be placed. The removal of the BioScaffold is performed by a Type IIB restriction enzyme (REase) that recognizes the BioScaffold but cuts into the surrounding sequences; therefore, the placement and removal of the BioScaffold allows the creation of seamless connections between arbitrary DNA sequences in cloning vectors. The BioScaffold contains a built-in red fluorescent protein (RFP) reporter; successful insertion of the BioScaffold is, thus, accompanied by gain of red fluorescence and its removal is manifested by disappearance of the red fluorescence.ConclusionsThe ability to perform targeted modifications of existing BioBrick circuits with BioScaffolds (1) simplifies and speeds up the iterative design-build-test process through direct reuse of existing circuits, (2) allows incorporation of sequences incompatible with BioBrick assembly into BioBrick circuits (3) removes scar sequences between standard biological parts, and (4) provides a route to adapt synthetic biology innovations to BioBrick assembly through the creation of new parts rather than new assembly standards or parts collections.

Dataset Information

A BioBrick compatible strategy for genetic modification of plants.

Unlabelled

Background

Results

Conclusions

Publications

A BioBrick compatible strategy for genetic modification of plants.

Similar Datasets

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