Ontology highlight
ABSTRACT:
SUBMITTER: De Ioannes P
PROVIDER: S-EPMC3538150 | biostudies-literature | 2012 Dec
REPOSITORIES: biostudies-literature
De Ioannes Pablo P Malu Shruti S Cortes Patricia P Aggarwal Aneel K AK
Cell reports 20121207 6
DNA ligase IV (LigIV) and Artemis are central components of the nonhomologous end-joining (NHEJ) machinery that is required for V(D)J recombination and the maintenance of genomic integrity in mammalian cells. We report here crystal structures of the LigIV DNA binding domain (DBD) in both its apo form and in complex with a peptide derived from the Artemis C-terminal region. We show that LigIV interacts with Artemis through an extended hydrophobic surface. In particular, we find that the helix α2 ...[more]