Transcriptional profiling by sequencing of oropharyngeal cancer.

ABSTRACT:

Objective

To compare full transcriptome expression levels of matched tumor and normal samples from patients with oropharyngeal carcinoma stratified by known tumor etiologic factors.

Patients and methods

Full transcriptome sequencing was analyzed for 10 matched tumor and normal tissue samples from patients with previously untreated oropharyngeal carcinoma. Transcriptomes were analyzed using massively parallel messenger RNA sequencing and validated using the NanoString nCounter system. Global gene expression levels were compared in samples grouped by smoking status and human papillomavirus status. This study was completed between June 10, 2010, and June 30, 2011.

Results

Global gene expression analysis indicated tumor tissue from former smokers grouped more closely to t

SUBMITTER: Laborde RR 

PROVIDER: S-EPMC3538409 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature