LCMV glycosylation modulates viral fitness and cell tropism.
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ABSTRACT:
SUBMITTER: Bonhomme CJ
PROVIDER: S-EPMC3538765 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Publications
LCMV glycosylation modulates viral fitness and cell tropism.
Bonhomme Cyrille J CJ Knopp Kristeene A KA Bederka Lydia H LH Angelini Megan M MM Buchmeier Michael J MJ
PloS one 20130107 1
The glycoprotein (GP) of arenaviruses is glycosylated at 11 conserved N-glycosylation sites. We constructed recombinant lymphocytic choriomeningitis virus (rLCMV) featuring either additions or deletions of these N-glycans to investigate their role in the viral life cycle. N-glycosylation at two sites, T87 and S97, were found to be necessary to rescue rLCMV. Three of nine successfully rescued mutants, S116A, T234A, and S373A, under selective pressures in either epithelial, neuronal, or macrophage ...[more]
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